Journal: Nature Communications
Article Title: Host albumin redirects Candida albicans metabolism to engage an alternative pathogenicity pathway
doi: 10.1038/s41467-025-61701-5
Figure Lengend Snippet: A Growth of different C. albicans isolates and deletion mutants in RPMI-1640 with or without albumin assessed by optical density measurements every 30 min for 45 h at 600 nm ( n = 3). B Representative microscopic images showing SC5314, efg1 ΔΔ/ cph1 ΔΔ, and 101 grown with and without albumin in the absence of host cells. Images were taken after 24 h at ×10 magnification (scale bar = 200 µm). C Cytotoxicity of A-431 cells infected with C. albicans strains at different multiplicities of infections (MOI) after 45 h post infection (hpi) with or without albumin ( n = 3). D Comparison of cytotoxicity induced to A-431 cells in a transwell system (indirect infection) to directly infected A-431 cells in presence or absence of albumin after 45 hpi ( n = 4). E Cytotoxicity of A-431 cells infected with albumin pre-incubated or non-albumin pre-incubated C. albicans strains. The pre-incubation took place for 0.5 h, 1 h, 2 h, or 3 h. The cytotoxicity measurements were carried out after 45 hpi, as a control served a direct infection with or without albumin ( n = 4). Cytotoxicity ( C – E ) was quantified by measuring the LDH activity in the supernatant. Bars represent the mean ± SEM, and dots represent the mean of the technical replicates of the individual experiments. Data were tested for significance using a two-way ANOVA with Holm-Šídák multiple comparisons test ( C – E ). P values are provided in the figure for significant comparisons. Source data are provided as a Source Data file.
Article Snippet: Transwell inserts (polycarbonate membrane inserts with 0.4 μm pore size; Corning) were placed in the well and filled with 250 μL of the desired C. albicans strain (1 × 10 5 yeast cells/transwell) in RPMI-1640 medium with or without 10 mg/mL albumin.
Techniques: Infection, Comparison, Incubation, Control, Activity Assay
